LM World

Chemical test for Myrrh Preliminary observations: Size and shape: rounded or irregular tears or masses Odour: agrreable and aromatic Colour: Externally reddish brown, internally brownish Taste: Aromatic, bitter and acrid Extra feature: Breaks with a brittle fracture, with somewhat granular, translucent surface Solubility: Insoluble in water, but soluble in chloroform and ether Chemical test: SI.No. Test Observation Inference 1. Triturate with water Yellowish brown emulsion Myrrh may be present 2. Extract small portion of powdered drug with solvent ether, evaporate the extract in such a way that the residue is left as a resinous thin film, pass the vapors of bromine over the film produced . A violet colour is produced Myrrh may be present 3. Dry etherial extract when treated with bromine vapours Reddish colour obtained M

Chemical test for Benzoin Objective:  To perform the chemical test for BENZOIN Biological source: It is a balsamic resin obtained from Styrax benzoin. Fam- Styraceae   Physical characteristics: Appearance           - Crystalline Colour                   - Greyish brown Odour                    - Aromatic and characteristics, balsamic Shape                    - Tear Taste                      - sweet and slightly acrid Sr. No. Test Observation Inference 1. Heat 0.5g of substance in dry test tube Evolve white fumes which condense to form white crystalline sublimate in the upper part of tube. Benzoin present 2. Alcoholic solution + water White milky solution formed Benzoin present 3. Alcoholic solution of litmus treat with litmus paper Acidic to litmus paper. Benzoin present 4. Heat 0

IDENTIFICATION TESTS FOR RESINOUS DRUGS Chemical test for Asafoetida Objective:  To perform the chemical test for ASAFOETIDA Biological source: It is the oleo resin obtained from roots and rhizomes of Ferula foetida. Fam- umbelliferae Physical characteristics: Appearance           - Crystalline Colour                   -Yellowish brown Odour                    - Intense penetrating and alliaceous odour Shape                    - Tear and masses Taste                      - Bitter acrid and alliaceous taste Solubility               - Partially soluble in alcohol Sr. No. Test Observation Inference 1. Triturate with water Yellowish orange  emulsion Asafoetida present 2. Treat fractured surface with with 50% nitric acid Green colour. Asafoetida present 3. Treat fractured surface with conc.sulphuric acid Red or redd

   Chemical test for Bees Wax Preliminary observations: Colour: Yellow to greyish brownsolid               White bees wax is yellowish white solid Odour: Yellow bees wax is agreeable and honey like White bees wax is faint and characteristics fracture Solubility: insoluble in water, soluble in alcohol and chloroform Extra feature: breaks with granular Chemical test: SI.No. Test Observation Inference 1. Heat with aqueous sodium hydroxide, boil, cool and acidify No turbidity Bees wax may be present 2. 1gm drug + 10ml alcohol, reflux for 1hour, stirr, Cloudy liquid between 60°C Bees wax may be present 3. 0.5gm of the wax boiled for 10mins in 100ml conical flask containing 20ml of aqueous caustic sod, cool and filtered, filterate is treated with hydrochloric acid Clear solution obtained Bees wax may be present

             Chemical test for Wool fat Preliminary observations: Colour: Whitish yellow Colour: Slightly characteristic Taste: Bland Solubility: Insoluble in water, but soluble in chloroform and ether Chemical test: SI.No. Test Observation Inference 1. 0.5gm wool fat + 5ml of chloroform. Add 1ml of acetic anhydride and 2 drops of  sulphuric acid Deep green colour Cholesterol may be present

Chemical test for Tragacanth Organoleptic test: Appearance: Crystalline                                                          tragacanth Colour: White or faint yellow                                                 tragacanth Odour: None                                                                           tragacanth Taste: Mucilaginous                                                                tragacanth Solubility: Insoluble in alcohol, partially soluble in water      tragacanth Chemical test: SI.No. Test Observation Inference 1. To 4ml of 0.5%w/v solution add  0.5ml of HCl and heat for 30minutes on water bath Divide the hydrolyzed product in 2 parts a)       1 st part=Add 1.5ml of NaOH solution to neutralize and add Fehling’s solution A and B in equal quantities(mixed before) and warm over water bath. b)       2 nd part= Add barium chloride solution Red ppt o