Chemical test for Bees Wax

   Chemical test for Bees Wax

Preliminary observations:

Colour: Yellow to greyish brownsolid

              White bees wax is yellowish white solid

Odour: Yellow bees wax is agreeable and honey like

White bees wax is faint and characteristics fracture

Solubility: insoluble in water, soluble in alcohol and chloroform

Extra feature: breaks with granular

Chemical test:

SI.No.	Test	Observation	Inference
1.	Heat with aqueous sodium hydroxide, boil, cool and acidify	No turbidity	Bees wax may be present
2.	1gm drug + 10ml alcohol, reflux for 1hour, stirr,	Cloudy liquid between 60°C	Bees wax may be present
3.	0.5gm of the wax boiled for 10mins in 100ml conical flask containing 20ml of aqueous caustic sod, cool and filtered, filterate is treated with hydrochloric acid	Clear solution obtained	Bees wax may be present