Chemical test for Bees Wax

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   Chemical test for Bees Wax
Preliminary observations:
Colour: Yellow to greyish brownsolid
              White bees wax is yellowish white solid
Odour: Yellow bees wax is agreeable and honey like
White bees wax is faint and characteristics fracture
Solubility: insoluble in water, soluble in alcohol and chloroform
Extra feature: breaks with granular

Chemical test:


SI.No.
Test
Observation
Inference

1.
Heat with aqueous sodium hydroxide, boil, cool and acidify
No turbidity
Bees wax may be present
2.
1gm drug + 10ml alcohol, reflux for 1hour, stirr,
Cloudy liquid between 60°C
Bees wax may be present
3.
0.5gm of the wax boiled for 10mins in 100ml conical flask containing 20ml of aqueous caustic sod, cool and filtered, filterate is treated with hydrochloric acid
Clear solution obtained
Bees wax may be present

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