Chemical test for Benzoin

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Chemical test for Benzoin

Objective: 
To perform the chemical test for BENZOIN

Biological source:
It is a balsamic resin obtained from Styrax benzoin.
Fam- Styraceae 

Physical characteristics:
Appearance           - Crystalline
Colour                   - Greyish brown
Odour                    - Aromatic and characteristics, balsamic
Shape                    - Tear
Taste                      - sweet and slightly acrid


Sr.
No.
Test
Observation
Inference
1.
Heat 0.5g of substance in dry test tube
Evolve white fumes which condense to form white crystalline sublimate in the upper part of tube.
Benzoin present
2.
Alcoholic solution + water
White milky solution formed

Benzoin present
3.
Alcoholic solution of litmus treat with litmus paper
Acidic to litmus paper.
Benzoin present
4.
Heat 0.5g with 10ml solution of potassium permangnate + 1 drop of conc. Sulphuric acid
Bitter almond smell of benzal- dehyde

Sumatra benzoin 
5.
2.5 g drug + 10 ml ether shake and warm . pour 2 to 3ml of this extract in porcellin dish containing 2 to 3 drops conc. Sulphuric acid
Deep reddish brown colour produced in porcellin dish.
Sumatra
Siam benzoin
6.
Truturate drug with alcohol, filter, filtrate+ferric chloride solution (alcoholic)
No bright green colour
Sumatra benzoin
7.
Heat small quantity of benzoin in test tube covering opening of test tube with glass slide cool the content and examine slide under microscope.
Crystals of cinnamic acid is observed
Benzoin conformed

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