IDENTIFICATION TEST FOR OIL AND FATS Chemical test for Castor oil
IDENTIFICATION
TEST FOR OIL AND FATS
Chemical
test for Castor oil
Preliminary observation:
Colour: Pale yellowish (or ) almost colourless, transparent viscous
liquid
Odour- Slight and characteristic
Taste- Bland, acrid, and nauseating
Solubility- Soluble in alcohol, miscible with chloroform, solvent
ether, glacial acetic acid and petroleum ether(40 ο -60ο)
Chemical test:
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SI.No
|
Test
|
Observation
|
Inference
|
|
1.
|
Add 5ml of light petroleum ether
(40 ο -60ο) to 10ml of castor oil
|
A clear solution results and on increasing
the petroleum ether about 15ml, turbid mixture will be obtained
|
Castor oil present
|
|
2.
|
Oil + equal volume of alcohol and cool to 0οC for 3hrs
|
A clear liquid is obtained
|
Castor oil is present.
|
Report:
Chemical
test for shark- liver oil
Preliminary observations:
Colour: Pale yellow to brownish yellow oil
Odour: Characteristic fishy but not acrid
Taste: Bland or fishy
Solubility: Miscible with solvent ether, chloroform and with light
petroleum (60 ο -80ο), slightly
soluble in alcohol.
Chemical test:
|
SI.No.
|
Test
|
Observation
|
Inference
|
|
1.
|
Dissolve 05 drops in 1ml of chloroform in a dry test tube and add 5
drop of concentarted sulphuric acid and shake well.
|
A pale violet colour is produced changing to purple brown or blue(due
to Vit. A)
|
Shark liver oil present
|
|
2.
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1ml oil + 10ml chloroform in dry test tube +saturated solution of antimony tri chloride
in chloroform solution
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Blue colour
|
Shark liver oil present
|
|
3.
|
5ml of shark liver oil + 5ml of benzene, centrifuge at 25οC
for 25mins
|
Clear solution with no precipitate
|
Shark liver oil present
|
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