ANTIANXIETY PROPERTY OF BASELLA ALBA LINN LEAVES EXTRACT IN EXPERIMENTAL ANIMALS - OBJECTIVES

 

2. OBJECTIVES

 

The literature reveals that anxiolytic activity on Basella alba leaves has not been reported. In view of this, flowers of Basella alba has been selected for anxiolytic activity on experimental animals.

The present study is therefore plan with the following objectives:

1.      To collect flowers and shade dry leaves of Basella alba, stored in well sealed cellophane bags and then coarsely powdered.

2.      To prepare various extracts (petroleum ether, chloroform, ethanol and aqueous extract) by successive extraction technique of the leaves of Basella alba.

3.      To carryout preliminary phytochemical analysis of crude extracts for the detection of the type of phytoconstituents present.

4.      To study the acute toxicity for determination of LD₅₀ of the extracts in albino mice.

5. Evaluation of various solvent extracts of Basella alba leaves for its anxiolytic activity by following models-
1. Elevated plus maze model.
2. Hole and board test.
3. Light and dark box test.
4. Open field test.