DEVELOPMENT AND EVALUATION OF FLOATING TABLETS OF CIPROFLOXACIN HCL - METHODOLOGY

 

METHODOLOGY

 

Materials

Table 1: Materials and sources

Sr.No.	Material	Source
1.	Ciprofloxacin HCL	RME’S College of Pharmacy, Gulbarga
2.	Sodium alginate	S.D. Fine Chem. Ltd. Mumbai.
3.	HPMC K4M	S.D. Fine Chem. Ltd. Mumbai.
4.	Carbopol 934 P	S.D. Fine Chem. Ltd. Mumbai.
5.	Sodium bi-carbonate	S.D. Fine Chem. Ltd. Mumbai.
6.	Magnesium sterate	S.D. Fine Chem. Ltd. Mumbai.
7.	PVP K30	S.D. Fine Chem. Ltd. Mumbai.
8.	Lactose	S.D. Fine Chem. Ltd. Mumbai.

 

Equipment:

Table 2: Equipment used

Sr.No.	Equipment	Source
1.	UV-visible spectrophotometer	PG instrument T80 model UV/VIS Spectrophotometer
2.	Electronic Balance	BT 220H -Shimadzu Digital Balance , Japan
3.	Magnetic stirrer	Remi Equipments, Mumbai
4.	Paddle type dissolution tester (USP-XXIII)	Campbell electronics, DR-6 Dissolution Apparatus
5.	Fourier Transformer Infrared (FTIR) Spectrophotometer	Shimadzu 8400-S, Japan.
6.	Stability chamber	Thermal Instrument & Equipment, Hyderabad
7.	Rotatory Tablet Punching Machine	Karnavati Mini Press

 

Evaluation of Ciprofloxacin HCL⁶⁵

Standard calibration curve for ciprofloxacin HCL in pH 1.2 M HCL buffer:

Stock Solution:

            Accurately weighted quantity of 100mg Ciprofloxacin HCL was dissolved in 0.1N Hcl using 100ml volumetric flask and volume was made upto 100ml distill water to produce 1mg/ml of solution.

Sub Stock Solution:

            From the above stock solution a series of dilution viz., 2, 4, 6, 8, 10mg/ml were prepared respectively. The absorbance was measured at 271 nm using PG instrument T₈₀ model UV/VIS spectrophotometer against reagent blank and graph was plotted as shown in table 4.

Preparation of Floating tablets of ciprofloxacin HCL⁶⁵

Preparation of Ciprofloxacin HCL Floating Tablets

            In the present work, wet granulation method has been employed to prepare floating tablets of Ciprofloxacin HCL with hydroxyl propyl methyl cellulose (HPMC K4M), carbopol 934 P and sodium alginate in four different polymer ratios as per the composition given in table No.4. Lactose was used as diluents along with sodium bicarbonate and citric acid as gas generating agents and PVP K30 (3%w/v solution) as binder, magnesium stearate was used as lubricant and talc as a glidant.

Preparation of Ciprofloxacin HCL Floating Tablets

            All the ingredients were accurately weighed, passed through sieve no.60 and transferred to clean porcelain mortar except magnesium stearate and talc. PVP K30 (3%w/v in iso propyle alcohol) binding solution was added to the powder mixture in small quantities, while mixing thoroughly after each addition until a coherent mass was formed and passed through sieve no.44. The wet granules were spread on a paper and dried in hot air oven at 55⁰C-60⁰C for 30 minutes. The above powder was blended with talc and magnesium stearete in the weight proportion as mention in table and compressed into floating tablets on rotatory punching machine (Karnavati Tablet Press) using 9mm concave punches.