CHEMISTRY OF GELATIN AND PEPTONE
CHEMISTRY OF
GELATIN AND PEPTONE
Gelatin and peptone are derived
proteins. Gelatin is a rich source of glyoine, but contains only traces of
tyrosine and lacks tryptophan, cystine and cystieine. Gelatin is a derived
protein obtained from collagen present in bones and white fibrous tissues. It
has a poor biological value, it is not heat coagulable and is precipitated by
half saturation with ammonium sulphate.
Peptones are the hydrolytic
products of proteoses. They are not heat coagulable, cannot be precipitated
either by half saturation or by full saturation with ammonium sulphate. If any
precipitate is formed it shows the contamination of primary or secondary proteoses.
Perform that following test
separately with gelatin and peptone and draw the proper inference.
1) Half Saturation test:
2) Full Saturation test:
3) Heat Coagulation test:
4) Colour reactions of
protein:
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