EFFECT OF DRUGS ON PERFUSED HEART OF FROG

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EFFECT OF DRUGS ON PERFUSED HEART OF FROG

 

Aim: To study the effect of Adrenaline and Acetytholinc on perfused heart of frog

 

Requires: Perfusion funnel, syme’s cannula perfusion fluid, Adrenaline (1. in 10000) and Acetylcholine (in 10,000)

 

Speed of the Drum: Slow speed with slow gear and pulleys S4 - Dl

 

Procedure: Frog’s heart is isolated and normal cardiogram is recorded by using perfusion- fluid. Few drops of Ademaline is added through the cannula and the effect is recorded. Then the solution in the cannula is washed out and normal cardiogram is recorded. Similarly the effect of actycholine is recorded.

 

Observation: When addrenoline is added there is increase in both rate and force of contraction, and Acetycholine in small doses decreases the rate and force of contraction, and in large doses If stops the heart in distole.

 

Inference: The adrenaline increases the rate and force of contraction of heart by accelerating the enzymatic and metabolic activities of cardiac tissue. The acetyicholine stops the heart in diastole by causing hyperpolarization.

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