EFFECT OF DRUGS ON PERFUSED HEART OF FROG
EFFECT OF
DRUGS ON PERFUSED HEART OF FROG
Aim: To study the effect of
Adrenaline and Acetytholinc on perfused heart of frog
Requires: Perfusion funnel, syme’s
cannula perfusion fluid, Adrenaline (1. in 10000) and Acetylcholine (in 10,000)
Speed of the
Drum: Slow
speed with slow gear and pulleys S4 - Dl
Procedure: Frog’s heart is isolated
and normal cardiogram is recorded by using perfusion- fluid. Few drops of Ademaline
is added through the cannula and the effect is recorded. Then the solution in
the cannula is washed out and normal cardiogram is recorded. Similarly the effect
of actycholine is recorded.
Observation: When addrenoline is added
there is increase in both rate and force of contraction, and Acetycholine in
small doses decreases the rate and force of contraction, and in large doses If
stops the heart in distole.
Inference: The adrenaline increases
the rate and force of contraction of heart by accelerating the enzymatic and
metabolic activities of cardiac tissue. The acetyicholine stops the heart in
diastole by causing hyperpolarization.
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