DETERMINATION OF PACKED CELL VOLUME

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DETERMINATION OF PACKED CELL VOLUME

 

Apparatus Required: Wintrobe haematocrit tube, Centrifuge, Fine Capillary droper, Oxalate bulb. Oxalated bulb (containing Potassium Oxal ate and ammonium Oxalate).

 

Method: Blood is drawn from a vein taking. all the precautions and is transferred to the oxalate bulb. Oxalate bulb is thoroughly mixed and wintrobe a filled with fine capillary dropper upto mark ‘O” (Zero), Tube is placed in a centrifuge and is revolved at speed 3000 rotation per minute (R.P.M.) for half an hour. After half an hour the packed cell volume is read directly from the markings on the tube.

 

Note: Normal Value 40-45%

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