DETERMINATION OF PACKED CELL VOLUME
DETERMINATION
OF PACKED CELL VOLUME
Apparatus
Required: Wintrobe
haematocrit tube, Centrifuge, Fine Capillary droper, Oxalate bulb. Oxalated
bulb (containing Potassium Oxal ate and ammonium Oxalate).
Method: Blood is drawn from a vein
taking. all the precautions and is transferred to the oxalate bulb. Oxalate
bulb is thoroughly mixed and wintrobe a filled with fine capillary dropper upto
mark ‘O” (Zero), Tube is placed in a centrifuge and is revolved at speed 3000
rotation per minute (R.P.M.) for half an hour. After half an hour the packed
cell volume is read directly from the markings on the tube.
Note: Normal Value 40-45%
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