DETERMINATION OF TITRABLE ACIDITY AND AMMONIA CONTENT IN URINE

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DETERMINATION OF TITRABLE ACIDITY

AND AMMONIA CONTENT IN URINE

 

 

Principal: The titrable acidity of urine is mainly due to acid phosphate and to a lesser extent due to weak organic acid. Titrable acidity can be determined by titrating urine with standard alkali with phenolphthalem indicator.

 

                NaH2PO4 + NaOH à NaHPO4 + H2O

 

                Since Calcium interferes during titration due to the precipitation as calcium phosphate. It is removed completely as calcium oxalate before estimation.

 

                Ammonia in urine arises from hydrolysis of glutamine (60%) and due to oxidative deamination (40%) in the Kidney.

 

                Ammonia is estimated by the formol titration method when neutral formaldhyde is added to solution. Containing ammonium salt’s H+ ions are librated which can be titrated with standard alkali. Hexamethylen tetramine is the other product in this reaction.

 

Procedure: A: Titration for titrable acidity

 

                Pipette 25ml urine into a 250 ml conical flask and add 2 spatula of potassium oxalate to precipitate calcium. Add 2 drops of phenolphthalein. Rotate the flask for 1-2 minutes and titrate against NaOH. Note the titer value (A ml) when a permanent pale pink colour appears preserve the contents for ammonia estimation.

 

                B: Titration for ammonia:

 

                Note initial reading of burette, Add 5 ml of neutralized formalin to the above flask, pink colour disappears as a result of liberation of H+. Titrate against NaOH until the pale pink colour appears. Record the titre value (Bml)repeat both titrations till concordant value are obtained.

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